FAK Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesFAK; FADK; FAK1; FRNK; pp125FAK; PTK2
ProductOrderF
Clone#10H7E9
Entrez GenelD5747
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human FAK expressed in E. Coli.
MW119kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
354-533
Catalog#: 30053
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Images
Western Blot
Figure 1: Western blot analysis using FAK mAb against FAK(AA: 354-533)-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using FAK mAb against FAK(AA: 354-533)-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of A549 cells using FAK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Immunofluorescence analysis of A549 cells using FAK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of Raji cells using FAK mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of Raji cells using FAK mouse mAb (green) and negative control (purple).
Product Overview
Description

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. Tissue specificity: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.RD: Focal adhesion kinase 1 (FAK) is a ubiquitously expressed non-receptor protein tyrosine kinase that is concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This cellular localization is directed by a

References (references)
References (references)1. J Biol Chem. 2009 Aug 21;284(34):22865-77.
2. Biochem Biophys Res Commun. 2009 Oct 16;388(2):301-5.