EZH2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesWVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
ProductOrderE
Clone#6G4F4
Entrez GenelD2146
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human EZH2 (AA: 1-194) expressed in E. Coli.
MW85.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-194
Catalog#: 30752
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using EZH2 mAb against human EZH2 (AA: 1-194) recombinant protein. (Expected MW is 48.5 kDa)
Figure 2:Western blot analysis using EZH2 mAb against human EZH2 (AA: 1-194) recombinant protein. (Expected MW is 48.5 kDa)
Western Blot
Figure 3:Western blot analysis using EZH2 mAb against HEK293 (1) and EZH2 (AA: 1-194)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using EZH2 mAb against HEK293 (1) and EZH2 (AA: 1-194)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using EZH2 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using EZH2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using EZH2 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using EZH2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EZH2 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EZH2 mouse mAb with DAB staining.
Product Overview
DescriptionThis gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
References (references)
References (references)Int J Gynecol Cancer. 2013 Jul;23(6):997-1005.
Cell Cycle. 2013 Jul 1;12(13):2113-9.