EZH1 Primary Antibody

Item Information
Catalog #
Size
Price
Description

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]

Product Overview
Entrez GenelD
2145
Aliases
KMT6B
Clone#
3B6B9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human EZH1 (AA: 135-294) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Nature. 2018 Jan 25;553(7689):506-510.
2.Pathology. 2016 Aug;48(5):467-82.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using EZH1 mAb against human EZH1 (AA: 135-294) recombinant protein. (Expected MW is 44.1 kDa)
Figure 2:Western blot analysis using EZH1 mAb against human EZH1 (AA: 135-294) recombinant protein. (Expected MW is 44.1 kDa)
Western Blot
Figure 3:Western blot analysis using EZH1 mAb against HEK293 (1) and EZH1 (AA: 135-294)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using EZH1 mAb against HEK293 (1) and EZH1 (AA: 135-294)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using EZH1 mouse mAb against HepG2 (1) and Hela (2) cell lysate.
Figure 4:Western blot analysis using EZH1 mouse mAb against HepG2 (1) and Hela (2) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using EZH1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using EZH1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.