ESR1 Primary Antibody

Item Information
Catalog #
Size
Price
Description
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.
Product Overview
Entrez GenelD
2099
Aliases
ER; ESR; Era; ESRA; ESTRR; NR3A1
Clone#
6A6E8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human ESR1 (AA: 2-185) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Tumour Biol. 2015 Aug;36(8):6349-59.
2.Breast Cancer Res. 2014 Dec 12;16(6):494.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)
Figure 2:Western blot analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)
Western Blot
Figure 3:Western blot analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.
Figure 4:Western blot analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 5:Immunofluorescence analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Immunofluorescence analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 7:Flow cytometric analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ESR1 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ESR1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ESR1 mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ESR1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.