ERN1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.

Product Overview

Entrez GenelD

2081

Aliases

IRE1; IRE1P; IRE1a; hIRE1p; FLJ30999; MGC163277; MGC163279; ERN1

Clone#

9F2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human ERN1(aa282-433) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Biochem Biophys Res Commun. 2004 Apr 30;317(2):390-6.
2. Mol Cell Biol. 2005 Sep;25(17):7522-33.
3. Science. 2007 Nov 9;318(5852):944-9.

Product Image

Western Blot

Figure 1: Western blot analysis using ERN1 mouse mAb against Raji (1), A431 (2), Jurkat (3), Hela(4) and HEK293 (5) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and stomach tissue (B), showing cytoplasmic localization using ERN1 mouse mAb with DAB staining.

Immunohistochemical analysis