Mouse Monoclonal Antibody to ERCC1
The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e49a3940edc0c429a62be1461b65d352993abede6aadc4b2eda8a5f10f03d1fd.jpg?v=1666705434)
![Figure 2:Western blot analysis using ERCC1 mAb against human ERCC1 (AA: 1-120) recombinant protein. (Expected MW is 15.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ffb51800c2b1428bd0f832470aa59c08644583bef358fc5e9e453a163ed636a9.jpg?v=1666705434)
![Figure 3:Western blot analysis using ERCC1 mAb against HEK293-6e (1) and ERCC1 (AA: 1-120)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e8bbe0a9fd4d927d7db418b87e908eebbc0068e5b189d51294c2f55279eb7aae.jpg?v=1666705434)
![Figure 4:Immunofluorescence analysis of Hela cells using ERCC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8ddb46bd4fa6096fb6bbcd1ee6ab56695766ea27b171794ed1be1b26b4493afd.jpg?v=1666705434)
![Figure 5:Flow cytometric analysis of A549 cells using ERCC1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32c3e487f28a99c7dc6b1d8bb4f7f0828e39c975e7d890186856d5ee08e0a42b.jpg?v=1666705434)
![Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ERCC1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/62742d89e79a3ae663ac2f2b9bd004f0ba337da11821cedcd60ad73f426a1466.jpg?v=1666705434)