Mouse Monoclonal Antibody to ERCC1

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Description

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.

Product Overview
Entrez GenelD
2067
Aliases
UV20; COFS4; RAD10
Clone#
5A2C3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human ERCC1 (AA: 1-297) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Indian J Pathol Microbiol. 2019 Jul-Sep;62(3):405-412. 2.Thorac Cancer. 2019 Mar;10(3):452-458.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ERCC1 mAb against human ERCC1 (AA: 1-297) recombinant protein. (Expected MW is 35.5 kDa)
Figure 2:Western blot analysis using ERCC1 mAb against human ERCC1 (AA: 1-297) recombinant protein. (Expected MW is 35.5 kDa)
Western Blot
Figure 3:Western blot analysis using ERCC1 mAb against HEK293-6e (1) and ERCC1 (AA: 1-297)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using ERCC1 mAb against HEK293-6e (1) and ERCC1 (AA: 1-297)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ERCC1 mouse mAb against NIH/3T3 (1), MCF-7 (2), Hela (3), SK-Br-3 (4), HepG2 (5), Raji (6), PC-3 (7), and A549 (8) cell lysate.
Figure 4:Western blot analysis using ERCC1 mouse mAb against NIH/3T3 (1), MCF-7 (2), Hela (3), SK-Br-3 (4), HepG2 (5), Raji (6), PC-3 (7), and A549 (8) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using ERCC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using ERCC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of A549 cells using ERCC1 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of A549 cells using ERCC1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.