ERBB3 Primary Antibody

Item Information
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Description

ERBB3, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian). It is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.

Product Overview
Entrez GenelD
2065
Aliases
HER3; c-erbB3
Clone#
2F9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant extracellular fragment of human ERBB3 (aa22-369) fused with hIgGFc tag expressed in HEK293 cells.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Cancer Sci. 2007 Sep;98(9):1498-503.
2. Breast Cancer Res. 2008;10(1):R2.
Product Image
Western Blot
Figure 1: Western blot analysis using ERBB3 mouse mAb against human ERBB3 (aa22-369)-hIgGFc trasfected HEK293 cell lysate (1).
Figure 1: Western blot analysis using ERBB3 mouse mAb against human ERBB3 (aa22-369)-hIgGFc trasfected HEK293 cell lysate (1).
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of HEK293 cells trasfected with extracellular ERBB3 (aa22-369)-hIgGFc using ERBB3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Confocal Immunofluorescence analysis of HEK293 cells trasfected with extracellular ERBB3 (aa22-369)-hIgGFc using ERBB3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.