ER-alpha Primary Antibody

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Description

The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.

Product Overview
Entrez GenelD
2099
Aliases
ER; ESR1; Era; ESRA
Clone#
2B7
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. Coli.
Formulation
Purified antibody in PBS containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Paech K. Science 1997, 277: 1508- 10.
2. Pertschuk LP,Cancer, 1996. 199677: 514- 9.
Product Image
Western Blot
Figure 1: Western blot analysis using ER-alpha mouse mAb against human breast carcinoma tissue lysate.
Figure 1: Western blot analysis using ER-alpha mouse mAb against human breast carcinoma tissue lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ER-alpha mouse mAb showing nuclear expression with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ER-alpha mouse mAb showing nuclear expression with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.