EPO Primary Antibody

Item Information

Catalog #

Size

Price

Description

Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.Tissue specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Product Overview

Entrez GenelD

2056

Aliases

EP; MVCD2; MGC138142; EPO

Clone#

4F11

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human EPO expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. J Negat Results Biomed. 2008 Nov 13;7:9.
2. J Biol Chem. 2009 Feb 13;284(7):4567-81.

Product Image

Western Blot

Figure 1: Western blot analysis using EPO mAb against HEK293 (1) and EPO(AA: 28-193)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of GC7901 cells using EPO mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

For Research Use Only. Not for use in diagnostic procedures.