EphB6 Primary Antibody

Item Information
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Description

EPH receptor B6 (EphB6), with 1006-amino acid protein(~ 110 kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Eph receptors and ephrin ligands are membrane-bound cell-cell communication molecules with well-defined functions in development. EphB6 is expressed both in a variety of embryonic and adult tissues. EphB6 is a unique member in the Eph family of receptor tyrosine kinases in that its kinase domain contains several alterations in conserved amino acids and is catalytically inactive. EphB6 can both positively and negatively regulate cell adhesion and migration and tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition.

Product Overview
Entrez GenelD
2051
Aliases
HEP; MGC129910; MGC129911
Clone#
8E7H12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of EphB6 expressed in E. Coli.
Formulation
Purified antibody in PBS containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Kazushige Ogawa, Hiroki Wada, Noriyoshi Okada J Cell Sci. 2006 Feb 1;119(Pt 3):559-70.
2. Hiroshi Matsuoka, Hiroya Obama, Meghan L. Kelly J Biol Chem. 2005 Aug 12;280(32):29355-63.

Product Image
Western Blot
Figure 1: Western blot analysis of Jurkat (1) and NIH/3T3 (2) cell lysate using EphB6 mouse mAb.
Figure 1: Western blot analysis of Jurkat (1) and NIH/3T3 (2) cell lysate using EphB6 mouse mAb.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma (left) and return carcinoma (right) tissue, showing cytoplasmic localization using EphB6 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma (left) and return carcinoma (right) tissue, showing cytoplasmic localization using EphB6 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.