EphB6 Primary Antibody

Item Information

Catalog #

Size

Price

Description

EPH receptor B6 (EphB6), with 1006-amino acid protein(~ 110 kDa), belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Eph receptors and ephrin ligands are membrane-bound cell-cell communication molecules with well-defined functions in development. EphB6 is expressed both in a variety of embryonic and adult tissues. EphB6 is a unique member in the Eph family of receptor tyrosine kinases in that its kinase domain contains several alterations in conserved amino acids and is catalytically inactive. EphB6 can both positively and negatively regulate cell adhesion and migration and tyrosine phosphorylation of the receptor by an Src family kinase acts as the molecular switch for the functional transition.

Product Overview

Entrez GenelD

2051

Aliases

HEP; MGC129910; MGC129911

Clone#

8E7H12

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of EphB6 expressed in E. Coli.

Formulation

Purified antibody in PBS containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Kazushige Ogawa, Hiroki Wada, Noriyoshi Okada J Cell Sci. 2006 Feb 1;119(Pt 3):559-70.
2. Hiroshi Matsuoka, Hiroya Obama, Meghan L. Kelly J Biol Chem. 2005 Aug 12;280(32):29355-63.

Product Image

Western Blot

Figure 1: Western blot analysis of Jurkat (1) and NIH/3T3 (2) cell lysate using EphB6 mouse mAb.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human bladder carcinoma (left) and return carcinoma (right) tissue, showing cytoplasmic localization using EphB6 mouse mAb with DAB staining.

For Research Use Only. Not for use in diagnostic procedures.