EPHB4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
EphB4: EPH receptor B4, also known as Htk, Myk1, Tyro11. Entrez Protein: NP_004435. It is a member of the Eph receptor family, which bind the ephrin ligand family. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The EphB4 protein binds to ephrin-B2 and plays an essential role in vascular development.
Product Overview
Entrez GenelD
2050
Aliases
HTK; MYK1; TYRO11
Clone#
5B5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant extracellular fragment of human EPHB4 fused with hIgGFc tag expressed in HEK293 cell line.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Br J Cancer. 2007 Apr 10;96(7):1083-91.
2. Dig Liver Dis. 2007 Aug;39(8):725-32.
2. Dig Liver Dis. 2007 Aug;39(8):725-32.
Product Image
Western Blot
Figure 1: Western blot analysis using EphB4 mouse mAb against extracellular domain of human EphB4 (aa16-539).
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of methanol-fixed HEK293 cells trasfected with EphB4-hIgGFc using EphB4 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.