EPCAM Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
ProductOrderE
Clone#7B5C10
Entrez GenelD4072
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human EPCAM (AA: 24-265) expressed in E. Coli.
MW35kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
24-265
Catalog#: 30630
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Images
Western Blot
Figure 1: Western blot analysis using EPCAM mAb against human EPCAM (AA: 24-265) recombinant protein. (Expected MW is 53.4 kDa)
Figure 1: Western blot analysis using EPCAM mAb against human EPCAM (AA: 24-265) recombinant protein. (Expected MW is 53.4 kDa)
Western Blot
Figure 2: Western blot analysis using EPCAM mAb against HEK293 (1) and EPCAM (AA: 24-265)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using EPCAM mAb against HEK293 (1) and EPCAM (AA: 24-265)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using EPCAM mouse mAb against A431 (1), MCF-7 (2) cell lysate.
Figure 3: Western blot analysis using EPCAM mouse mAb against A431 (1), MCF-7 (2) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of A431 cells using EPCAM mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of A431 cells using EPCAM mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EPCAM mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EPCAM mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using EPCAM mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using EPCAM mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThis gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
References (references)
References (references)1. BMC Cancer. 2012 Oct 30;12:501.
2. Am J Surg Pathol. 2012 Dec;36(12):1809-16.