ENO2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Product Overview
Entrez GenelD
2026
Aliases
NSE
Clone#
5D3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human ENO2 (AA: 251-433) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Neurology. 2011 Aug 16;77(7):623-30.
2.Lung Cancer. 2011 Feb;71(2):224-8.
2.Lung Cancer. 2011 Feb;71(2):224-8.
Product Image
Western Blot
Figure 1: Western blot analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa)
Western Blot
Figure 2: Western blot analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ENO2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ENO2 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.