EMD Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesSTA; EDMD; LEMD5
ProductOrderE
Clone#8F5G2
Entrez GenelD2010
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human *** (AA: 1-222) expressed in E. Coli.
MW29kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-222
Catalog#: 30790
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)
Figure 2:Western blot analysis using EMD mAb against human EMD (AA: 1-222) recombinant protein. (Expected MW is 51.1 kDa)
Western Blot
Figure 3:Western blot analysis using EMD mAb against HEK293 (1) and EMD (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using EMD mAb against HEK293 (1) and EMD (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of A549 cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of A549 cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using EMD mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of K562 cells using EMD mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of K562 cells using EMD mouse mAb (green) and negative control (red).
Flow cytometric
Figure 7:Flow cytometric analysis of A549 cells using EMD mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of A549 cells using EMD mouse mAb (green) and negative control (red).
Product Overview
DescriptionEmerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
References (references)
References (references)1.Histopathology. 2009 Apr;54(5):571-9.
2.J Cell Biol. 2007 Sep 10;178(6):897-904.