EIF5A Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesEIF-5A; EIF5A1; eIF5AI
ProductOrderE
Clone#4E10G8
Entrez GenelD1984
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human EIF5A (AA: full(1-154)) expressed in E. Coli.
MW16.8kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
full(1-154)
Catalog#: 30713
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Images
Western Blot
Figure 1: Western blot analysis using EIF5A mAb against human EIF5A (AA: full(1-154)) recombinant protein. (Expected MW is 42.8 kDa)
Figure 1: Western blot analysis using EIF5A mAb against human EIF5A (AA: full(1-154)) recombinant protein. (Expected MW is 42.8 kDa)
Western Blot
Figure 2: Western blot analysis using EIF5A mAb against HEK293 (1) and EIF5A (AA: full(1-154))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using EIF5A mAb against HEK293 (1) and EIF5A (AA: full(1-154))-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using EIF5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3:Immunofluorescence analysis of Hela cells using EIF5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using EIF5A mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Hela cells using EIF5A mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF5A mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF5A mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using EIF5A mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using EIF5A mouse mAb with DAB staining.
Western Blot
Figure 8:Western blot analysis using EIF5A mouse mAb against Raji (1) cell lysate.
Figure 8:Western blot analysis using EIF5A mouse mAb against Raji (1) cell lysate.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

EIF5A (eukaryotic translation initiation factor 5A) is a protein-coding gene. Diseases associated with EIF5A include lung adenocarcinoma, and intrahepatic cholangiocarcinoma, and among its related super-pathways are Post-translational protein modification and Apoptotic Pathways in Synovial Fibroblasts. GO annotations related to this gene include ribosome binding and RNA binding. An important paralog of this gene is EIF5AL1.

References (references)
References (references)1. Int J Cancer. 2011 Jul 1;129(1):143-50.
2. Int J Cancer. 2010 Aug 15;127(4):968-76.