EIF4E Primary Antibody
Item Information
Catalog #
Size
Price
Description
eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.
Product Overview
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Image
Western Blot

Figure 1: Western blot analysis using EIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.
Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using EIF4E mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using EIF4E mouse mAb with DAB staining.
Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of GC-7901 cells using EIF4E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric

Figure 5: Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).
Elisa

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.