EIF2AK2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.
Product Overview
Entrez GenelD
5610
Aliases
PKR; PRKR; EIF2AK1
Clone#
3A10F7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human EIF2AK2 (AA: 329-551) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Alzheimers Dis. 2010;21(4):1217-31.
2. Mol Cells. 2011 Aug;32(2):167-72.
2. Mol Cells. 2011 Aug;32(2):167-72.
Product Image
Western Blot
Figure 1: Western blot analysis using EIF2AK2 mAb against human EIF2AK2 (AA: 329-551) recombinant protein. (Expected MW is 51.7 kDa)
Western Blot
Figure 2: Western blot analysis using EIF2AK2 mAb against HEK293 (1) and EIF2AK2 (AA: 329-551)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using EIF2AK2 mouse mAb against A431 (1), MCF-7 (2), PC-12 (3) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of A431 cells using EIF2AK2 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.