EI2BG
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
8891
Aliases
EIF2B3;EIF-2B; EIF2Bgamma
Clone#
1B9C12
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human EI2BG expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
References
1.Brain Dev. 2016 May;38(5):507-15.
2.PLoS One. 2015 Mar 11;10(3):e0118001.
2.PLoS One. 2015 Mar 11;10(3):e0118001.
Product Image
Western Blot
Figure 1:Western blot analysis using EI2BG mouse mAb against K562 (1), Hela (2),MCF-7 (3) and HL-60 (4) cell lysate.
Immunohistochemical analysis
Figure 2:Immunofluorescence analysis of Hela cells using EI2BG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 3:Flow cytometric analysis of HL-60 cells using EI2BG mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded liver tissues using EI2BG mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded kidney tissues using EI2BG mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.