EGFR Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesERBB; HER1; mENA; ERBB1; PIG61
ProductOrderE
Clone#5E10D3
Entrez GenelD1956
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli.
MW175kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
693-893
Catalog#: 30551
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Images
Western Blot
Figure 1: Western blot analysis using EGFR mAb against human EGFR recombinant protein. (Expected MW is 48.2 kDa)
Figure 1: Western blot analysis using EGFR mAb against human EGFR recombinant protein. (Expected MW is 48.2 kDa)
Western Blot
Figure 2: Western blot analysis using EGFR mAb against HEK293 (1) and EGFR (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using EGFR mAb against HEK293 (1) and EGFR (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.
Figure 3: Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
References (references)
References (references)1. J Immunol. 2012 Dec 1;189(11):5230-9.
2. J Biol Chem. 2012 Oct 12;287(42):35201-11.