EGFR Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | ERBB; HER1; mENA; ERBB1; PIG61 |
| ProductOrder | E |
| Clone# | 5E10D3 |
| Entrez GenelD | 1956 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG2b |
| Immunogen | Purified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli. |
| MW | 175kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 693-893 | |
Western Blot
Figure 1: Western blot analysis using EGFR mAb against human EGFR recombinant protein. (Expected MW is 48.2 kDa)
Western Blot
Figure 2: Western blot analysis using EGFR mAb against HEK293 (1) and EGFR (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);