EGF Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesURG; HOMG4; EGF
ProductOrderE
Clone#4E11
Entrez GenelD1950
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human EGF expressed in E. Coli.
MW134kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
971-1023
Catalog#: 30015
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Images
Western Blot
Figure 1: Western blot analysis using EGF mAb against EGF(AA: 971-1023)-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using EGF mAb against EGF(AA: 971-1023)-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using EGF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using EGF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Overview
Description

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

References (references)
References (references)1. Biochem J. 1992 Dec 1;288 ( Pt 2):395-405.
2. Oncogene. 2000 Mar 16;19(12):1509-18.
3. Nature. 2002 Mar 14;416(6877):183-7.
4. Radiat Res. 2003 Apr;159(4):439-52.