EGF Primary Antibody

Item Information

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Size

Price

Description

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Product Overview

Entrez GenelD

1950

Aliases

URG; HOMG4; EGF

Clone#

4E11

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human EGF expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Biochem J. 1992 Dec 1;288 ( Pt 2):395-405.
2. Oncogene. 2000 Mar 16;19(12):1509-18.
3. Nature. 2002 Mar 14;416(6877):183-7.
4. Radiat Res. 2003 Apr;159(4):439-52.

Product Image

Western Blot

Figure 1: Western blot analysis using EGF mAb against EGF(AA: 971-1023)-hIgGFc transfected HEK293 cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of NIH/3T3 cells using EGF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

For Research Use Only. Not for use in diagnostic procedures.