EEF2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. (provided by RefSeq)

Product Overview

Entrez GenelD

1938

Aliases

EF2; EEF-2; EEF2

Clone#

5B6

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human EEF2 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Mol Cell Biol. 2008 Dec;28(23):7050-65.
2. Am J Physiol Regul Integr Comp Physiol. 2009 Feb;296(2):R326-33.

Product Image

Western Blot

Figure 1: Western blot analysis using EEF2 mouse mAb against HepG2 (1), Hela (2), HEK293 (3) and A431 (4) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and kidney cancer tissues (right) using EEF2 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and tonsil tissues (right) using EEF2 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of 3T3-L1 cells using EEF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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