EDN1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants.

Product Overview

Entrez GenelD

1906

Aliases

ET1; QME; PPET1; ARCND3; HDLCQ7

Clone#

4E6E5

Host / Isotype

Mouse / Mouse IgG2a

Species Reactivity

Human, Mouse

Immunogen

Purified recombinant fragment of human EDN1 (AA: 18-212) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1，BMC Res Notes. 2021 May 19;14(1):194.
2，Respir Res. 2021 May 13;22(1):148.

Product Image

Western Blot

Figure 2:Western blot analysis using EDN1 mAb against human EDN1 (AA: 18-212) recombinant protein. (Expected MW is 25 kDa)

Western Blot

Figure 3:Western blot analysis using EDN1 mAb against HEK293-6e (1) and human EDN1 (AA: 18-212)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis

Figure 4:Immunofluorescence analysis of Hela cells using EDN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis