EDN1
Item Information
Catalog #
Size
Price
Description
This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
1906
Aliases
ET1; QME; PPET1; ARCND3; HDLCQ7
Clone#
4E6E5
Host / Isotype
Mouse / Mouse IgG2a
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human EDN1 (AA: 18-212) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1，BMC Res Notes. 2021 May 19;14(1):194.
2，Respir Res. 2021 May 13;22(1):148.
2，Respir Res. 2021 May 13;22(1):148.
Product Image
Western Blot
Figure 2:Western blot analysis using EDN1 mAb against human EDN1 (AA: 18-212) recombinant protein. (Expected MW is 25 kDa)
Western Blot
Figure 3:Western blot analysis using EDN1 mAb against HEK293-6e (1) and human EDN1 (AA: 18-212)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using EDN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using EDN1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HepG2 cells using EDN1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Flow cytometric analysis of NIH3T3 cells using EDN1 mouse mAb (green) and negative control (red).
Elisa
Figure 7:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
For Research Use Only. Not for use in diagnostic procedures.