EDN1
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | ET1; QME; PPET1; ARCND3; HDLCQ7 |
| Clone# | 4E6E5 |
| Entrez GenelD | 1906 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Immunogen | Purified recombinant fragment of human EDN1 (AA: 18-212) expressed in E. Coli. |
| MW | 24.4KDa |
| Species Reactivity | Human, Mouse |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 18-212 | |
Western Blot
Figure 2:Western blot analysis using EDN1 mAb against human EDN1 (AA: 18-212) recombinant protein. (Expected MW is 25 kDa)
Western Blot
Figure 3:Western blot analysis using EDN1 mAb against HEK293-6e (1) and human EDN1 (AA: 18-212)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using EDN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using EDN1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HepG2 cells using EDN1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Flow cytometric analysis of NIH3T3 cells using EDN1 mouse mAb (green) and negative control (red).
Elisa
Figure 7:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)