EDN1

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesET1; QME; PPET1; ARCND3; HDLCQ7
Clone#4E6E5
Entrez GenelD1906
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG2a
ImmunogenPurified recombinant fragment of human EDN1 (AA: 18-212) expressed in E. Coli.
MW24.4KDa
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
18-212
Catalog#: 32283
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Images
Western Blot
Figure 2:Western blot analysis using EDN1 mAb against human EDN1 (AA: 18-212) recombinant protein. (Expected MW is 25 kDa)
Figure 2:Western blot analysis using EDN1 mAb against human EDN1 (AA: 18-212) recombinant protein. (Expected MW is 25 kDa)
Western Blot
Figure 3:Western blot analysis using EDN1 mAb against HEK293-6e (1) and human EDN1 (AA: 18-212)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using EDN1 mAb against HEK293-6e (1) and human EDN1 (AA: 18-212)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using EDN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using EDN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using EDN1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using EDN1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HepG2 cells using EDN1 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HepG2 cells using EDN1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Flow cytometric analysis of NIH3T3 cells using EDN1 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of NIH3T3 cells using EDN1 mouse mAb (green) and negative control (red).
Elisa
Figure 7:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 7:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Product Overview
Description

This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants.

References (references)
References (references)1,BMC Res Notes. 2021 May 19;14(1):194.
2,Respir Res. 2021 May 13;22(1):148.