Dynamin-2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

Dynamin-2 (Dyn2), with 870-amino acid protein (about 95kDa), belongs to the dynamin family. Dynamin-1 (neuron-specific), dynamin-2 (ubiquitously expressed), and dynamin-3 (expressed only in the testis, brain, and lung), constitute the dynamin family. Members of the dynamin family are GPTase, microtubule-associated proteins which are involved in endocytosis, synaptic transmission and neurogenesis. Dynamin 1 is expressed exclusively in neurons, Dynamin 2 is ubiquitously expressed and Dynamin 3 is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.

Product Overview

Entrez GenelD

1785

Aliases

DYN2; DYNII; CMTDI1; CMTDIB; DI-CMTB; DNM2

Clone#

5E4C2F3

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of Dynamin-2 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Annie Quan and Phillip J. Robinson. Methods Enzymol. 2005; 404:556-69.
2. Jiyun Yoo, Moon-Jin Jeong, Byoung-Mog Kwon. J. Biol. Chem., Mar 2002; 277: 11904 - 11909

Product Image

Western Blot

Figure 1: Western blot analysis using Dynamin2 mouse mAb against truncated Dynamin-2 recombinant protein (1), SKN-SH cell lysate (2) and NIH/3T3 cell lysate (3).

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human cerebrum tissue (left) and myelencephalon tissue (right), showing cytoplasmic localization using Dynamin2 mouse mAb with DAB staining.

For Research Use Only. Not for use in diagnostic procedures.