DNTT Primary Antibody

Item Information
Catalog #
Size
Price
Description
This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Product Overview
Entrez GenelD
1791
Aliases
TDT
Clone#
4B10A6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human DNTT (AA: 52-192) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mod Pathol. 2013 Oct;26(10):1338-45.
2.Haematologica. 2006 Aug;91(8):1139-40.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using DNTT mAb against human DNTT (AA: 52-192) recombinant protein. (Expected MW is 42 kDa)
Figure 2:Western blot analysis using DNTT mAb against human DNTT (AA: 52-192) recombinant protein. (Expected MW is 42 kDa)
Western Blot
Figure 3:Western blot analysis using DNTT mAb against HEK293 (1) and DNTT (AA: 52-192)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using DNTT mAb against HEK293 (1) and DNTT (AA: 52-192)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using DNTT mouse mAb against Raji (1), A549 (2), Hela (3), and PC-12 (4) cell lysate.
Figure 4:Western blot analysis using DNTT mouse mAb against Raji (1), A549 (2), Hela (3), and PC-12 (4) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of *** cells using Hela mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of *** cells using Hela mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.