DNMT3L Primary Antibody
Item Information
Catalog #
Size
Price
Description
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
29947
Aliases
N
Clone#
2D10H11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human DNMT3L (AA: 147-386) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Clin Cancer Res. 2010 May 15;16(10):2751-9.
2.Epigenetics. 2009 Jul 1;4(5):322-9.
2.Epigenetics. 2009 Jul 1;4(5):322-9.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using DNMT3L mAb against human DNMT3L (AA: 147-386) recombinant protein. (Expected MW is 53.9 kDa)
Western Blot
Figure 3:Western blot analysis using DNMT3L mAb against HEK293 (1) and DNMT3L (AA: 147-386)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using DNMT3L mouse mAb (green) and negative control (red).
Flow cytometric
Figure 5:Flow cytometric analysis of HepG2 cells using DNMT3L mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.