DNMT3L Primary Antibody
Item Information
Catalog #
Size
Price
Description
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
Immunogen
Purified recombinant fragment of human DNMT3L (AA: 147-386) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using DNMT3L mAb against human DNMT3L (AA: 147-386) recombinant protein. (Expected MW is 53.9 kDa)
Western Blot

Figure 3:Western blot analysis using DNMT3L mAb against HEK293 (1) and DNMT3L (AA: 147-386)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric

Figure 4:Flow cytometric analysis of Hela cells using DNMT3L mouse mAb (green) and negative control (red).
Flow cytometric

Figure 5:Flow cytometric analysis of HepG2 cells using DNMT3L mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.