DNMT3A Primary Antibody
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Description
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using DNMT3A mAb against human DNMT3A (AA: 46-180) recombinant protein. (Expected MW is 40 kDa)
Western Blot

Figure 3:Western blot analysis using DNMT3A mAb against HEK293 (1) and DNMT3A (AA: 46-180)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis

Figure 4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DNMT3A mouse mAb with DAB staining.
Flow cytometric

Figure 5:Flow cytometric analysis of Hela cells using DNMT3A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.