DNMT3A Primary Antibody
Item Information
Catalog #
Size
Price
Description
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product Overview
Entrez GenelD
1788
Aliases
TBRS; DNMT3A2; M.HsaIIIA
Clone#
4G5F11
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human DNMT3A (AA: 46-180) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2014 Jun 17;9(6):e93353.
2.Asian Pac J Cancer Prev. 2013;14(10):5713-8.
2.Asian Pac J Cancer Prev. 2013;14(10):5713-8.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using DNMT3A mAb against human DNMT3A (AA: 46-180) recombinant protein. (Expected MW is 40 kDa)
Western Blot
Figure 3:Western blot analysis using DNMT3A mAb against HEK293 (1) and DNMT3A (AA: 46-180)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DNMT3A mouse mAb with DAB staining.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using DNMT3A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.