DNMT1 Primary Antibody
Item Information
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Price
Description
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
1786
Aliases
AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Clone#
2D10G8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human DNMT1 (AA: 1317-1616) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.J Biol Chem. 2013 Jul 5;288(27):19673-84.
2.Int J Oncol. 2013 Jul;43(1):228-36.
2.Int J Oncol. 2013 Jul;43(1):228-36.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using DNMT1 mAb against human DNMT1 (AA: 1317-1616) recombinant protein. (Expected MW is 59.3 kDa)
Western Blot
Figure 3:Western blot analysis using DNMT1 mAb against HEK293 (1) and DNMT1 (AA: 1317-1616)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using DNMT1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DNMT1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.