DNMT1 Primary Antibody
Item Information
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Price
Description
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
1786
Aliases
AIM; DNMT; MCMT; CXXC9; HSN1E
Clone#
7C11A2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of human DNMT1 (AA: 1448-1594) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. PLoS One. 2011;6(11):e27684.
2. J Exp Clin Cancer Res. 2011 Oct 17;30:98.
2. J Exp Clin Cancer Res. 2011 Oct 17;30:98.
Product Image
Western Blot
Figure 1: Western blot analysis using DNMT1 mAb against human DNMT1 recombinant protein. (Expected MW is 42.6 kDa)
Western Blot
Figure 2: Western blot analysis using DNMT1 mAb against HEK293 (1) and DNMT1 (AA: 1448-1594)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using IL2RA mouse mAb against Jurkat (1), Cos7 (2), HCT116 (3) and NTERA-2 (4) cell lysate.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using DNMT1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.