DNM1L Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated.
Product Overview
Entrez GenelD
10059
Aliases
DLP1; DRP1; DVLP; EMPF; VPS1; DYMPLE; HDYNIV; DYNIV-11
Clone#
4A4C7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human DNM1L (AA: 69-213) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. PLoS One. 2012;7(9):e45319.
2. Circ Res. 2012 May 25;110(11):1484-97.
2. Circ Res. 2012 May 25;110(11):1484-97.
Product Image
Western Blot
Figure 1: Western blot analysis using DNM1L mAb against human DNM1L recombinant protein. (Expected MW is 41.8 kDa)
Western Blot
Figure 2: Western blot analysis using DNM1L mAb against HEK293 (1) and DNM1L (AA: 69-213)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using DNM1L mouse mAb against PC-12 (1), LNCAP (2) and NIH/3T3 (3) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using DNM1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 5: Flow cytometric analysis of HEK293 cells using DNM1L mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.