DLL3 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene.

Product Overview

Entrez GenelD

10683

Aliases

SCDO1

Clone#

6H4C4

Host / Isotype

Mouse / Mouse IgG2a

Species Reactivity

Human, Rat

Immunogen

Purified recombinant fragment of human DLL3 (AA: EXTRA(27-226)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Cancer Sci . 2021 Aug;112(8):2984-2992.
2,Lung Cancer . 2019 Dec;138:102-108.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using DLL3 mAb against human DLL3 (AA: EXTRA(27-226)) recombinant protein. (Expected MW is 26.5 kDa)

Western Blot

Figure 3:Western blot analysis using DLL3 mAb against HEK293-6e (1) and DLL3 (AA: EXTRA(27-226))-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using DLL3 mouse mAb against Hela (1)cell lysate.

Immunohistochemical analysis

Figure 5:Immunofluorescence analysis of Hela cells using DLL3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis