DKK3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.
Product Overview
Entrez GenelD
27122
Aliases
RIG; REIC
Clone#
8A5C6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human DKK3 (AA: 91-350 ) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.Exp Dermatol. 2011 Mar;20(3):273-7.
2.Thromb Haemost. 2011 Jan;105(1):72-80.
2.Thromb Haemost. 2011 Jan;105(1):72-80.
Product Image
Western Blot
Figure 1: Western blot analysis using DKK3 mAb against human DKK3 recombinant protein. (Expected MW is 54.4 kDa)
Western Blot
Figure 2: Western blot analysis using DKK3 mAb against HEK293 (1) and DKK3 (AA: 91-350)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded brain tissues using DKK3 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.