DDX58 Primary Antibody
Item Information
Catalog #
Size
Price
Description
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.
Product Overview
Entrez GenelD
23586
Aliases
RIGI; RIG-I; RLR-1; SGMRT2
Clone#
4G1B6
Host / Isotype
Mouse / IgM
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human DDX58 (AA: 789-925) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2014 Jun 11;9(6):e99610.
2.J Pathol. 2014 Jul;233(3):258-68.
2.J Pathol. 2014 Jul;233(3):258-68.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using DDX58 mAb against human DDX58 (AA: 789-925) recombinant protein. (Expected MW is 42 kDa)
Western Blot
Figure 3:Western blot analysis using DDX58 mAb against HEK293 (1) and DDX58 (AA: 789-925)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using DDX58 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of MCF-7 cells using DDX58 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using DDX58 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.