DDX3X Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
1654
Aliases
DBX; DDX3; HLP2; DDX14; CAP-Rf
Clone#
3B9E3
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human, Mouse, Monkey, Rat
Immunogen
Purified recombinant fragment of human DDX3X (AA: 518-661) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2013;8(3):e59445.
2.Oral Dis. 2014 Jan;20(1):76-83.
2.Oral Dis. 2014 Jan;20(1):76-83.
Product Image
Western Blot
Figure 2:Western blot analysis using DDX3X mAb against human DDX3X (AA: 518-661) recombinant protein. (Expected MW is 41.4 kDa)
Western Blot
Figure 3:Western blot analysis using DDX3X mAb against HEK293 (1) and DDX3X (AA: 518-661)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using DDX3X mouse mAb against Hela (1), NIH3T3 (2), C6 (3), COS7 (4), A431 (5), and HEK293 (6) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using DDX3X mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using DDX3X mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 7:Flow cytometric analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).
Flow cytometric
Figure 8:Flow cytometric analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DDX3X mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using DDX3X mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
For Research Use Only. Not for use in diagnostic procedures.