DDX20 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesDP103; GEMIN3
ProductOrderD
Clone#1C9F6
Entrez GenelD11218
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human DDX20 (AA: 725-824) expressed in E. Coli.
MW92.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
725-824
Catalog#: 30899
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using DDX20 mAb against human DDX20 (AA: 725-824) recombinant protein. (Expected MW is 38.4 kDa)
Figure 2:Western blot analysis using DDX20 mAb against human DDX20 (AA: 725-824) recombinant protein. (Expected MW is 38.4 kDa)
Western Blot
Figure 3:Western blot analysis using DDX20 mAb against HEK293 (1) and DDX20 (AA: 725-824)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using DDX20 mAb against HEK293 (1) and DDX20 (AA: 725-824)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using DDX20 mouse mAb against Jurkat (1), Hela (2), NTERA-2 (3), HL7702 (4), K562 (5), and C6 (6) cell lysate.
Figure 4:Western blot analysis using DDX20 mouse mAb against Jurkat (1), Hela (2), NTERA-2 (3), HL7702 (4), K562 (5), and C6 (6) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of HeLa cells using DDX20 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of HeLa cells using DDX20 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of HeLa cells using DDX20 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HeLa cells using DDX20 mouse mAb (green) and negative control (red).
Product Overview
DescriptionDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.
References (references)
References (references)1.J Clin Invest. 2014 Sep;124(9):3807-24.
2.J Biol Chem. 1999 Jul 2;274(27):19136-44.