DDX20 Primary Antibody
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human DDX20 (AA: 725-824) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Rat|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using DDX20 mAb against human DDX20 (AA: 725-824) recombinant protein. (Expected MW is 38.4 kDa)
Figure 3:Western blot analysis using DDX20 mAb against HEK293 (1) and DDX20 (AA: 725-824)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using DDX20 mouse mAb against Jurkat (1), Hela (2), NTERA-2 (3), HL7702 (4), K562 (5), and C6 (6) cell lysate.
Figure 5:Immunofluorescence analysis of HeLa cells using DDX20 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of HeLa cells using DDX20 mouse mAb (green) and negative control (red).
|Description||DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs. |
|References (references)||1.J Clin Invest. 2014 Sep;124(9):3807-24. |
2.J Biol Chem. 1999 Jul 2;274(27):19136-44.