DDIT3 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCHOP; CEBPZ; CHOP10; CHOP-10; GADD153
ProductOrderD
Clone#4E8E9
Entrez GenelD1649
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG2a
ImmunogenPurified recombinant fragment of human DDIT3 (AA: 87-192) expressed in E. Coli.
MW19.2kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
87-192
Catalog#: 30633
Inquire
Contact us to order
1-866-339-0871
510-740-3625
info@promab.com
Images
Western Blot
Figure 1: Western blot analysis using DDIT3 mAb against human DDIT3 (AA: 87-192) recombinant protein. (Expected MW is 38.2 kDa)
Figure 1: Western blot analysis using DDIT3 mAb against human DDIT3 (AA: 87-192) recombinant protein. (Expected MW is 38.2 kDa)
Western Blot
Figure 2: Western blot analysis using DDIT3 mAb against HEK293 (1) and DDIT3 (AA: 87-192)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using DDIT3 mAb against HEK293 (1) and DDIT3 (AA: 87-192)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of MCF-7 cells using DDIT3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3: Immunofluorescence analysis of MCF-7 cells using DDIT3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of MCF-7 cells using DDIT3 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of MCF-7 cells using DDIT3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DDIT3 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using DDIT3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using DDIT3 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using DDIT3 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

References (references)
References (references)1. J Exp Clin Cancer Res. 2010 May 23;29:54.
2. J Biol Chem. 2010 Jun 25;285(26):19710-9.
Please view FAQs here