DCLK2

Item Information
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Description

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified.

Product Overview
Entrez GenelD
166614
Aliases
CL2; DCK2; CLIK2; DCDC3; CLICK2; DCDC3B; DCAMKL2; CLICK-II
Clone#
3H1G4
Host / Isotype
Mouse / Mouse IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human DCLK2 (AA: 652-766) expressed in mammalian.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Biol Chem. 2005 Mar 4;280(9):8531-43.
2.Cell Mol Biol Lett. 2008;13(4):614-20.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using DCLK2 mAb against human DCLK2 (AA: 652-766) recombinant protein. (Expected MW is 42.8 kDa)
Figure 2:Western blot analysis using DCLK2 mAb against human DCLK2 (AA: 652-766) recombinant protein. (Expected MW is 42.8 kDa)
Immunofluorescence analysis
Figure 3:Flow cytometric analysis of U937 cells using DCLK2 mouse mAb (green) and negative control (red).
Figure 3:Flow cytometric analysis of U937 cells using DCLK2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded human brain tissues using DCLK2 mouse mAb with DAB staining.
Figure 4:Immunohistochemical analysis of paraffin-embedded human brain tissues using DCLK2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.