CYLD Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Product Overview
Entrez GenelD
1540
Aliases
EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2
Clone#
5F7F4
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CYLD (AA: 204-358) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Biol Rep. 2017 Oct;44(5):391-397. 2.Cell Biochem Funct. 2016 Oct;34(7):465-468.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CYLD mAb against human CYLD (AA: 204-358) recombinant protein. (Expected MW is 42.7 kDa)
Western Blot
Figure 3:Western blot analysis using CYLD mAb against HEK293 (1) and CYLD (AA: 204-358)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using CYLD mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.