Mouse Monoclonal Antibody to CXCL9
Item Information
Catalog #
Size
Price
Description
This antimicrobial gene encodes a protein thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils.
Product Overview
Entrez GenelD
4283
Aliases
CMK; MIG; Humig; SCYB9; crg-10
Clone#
5H10F12
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human CXCL9 (AA: 23-125) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,bioRxiv. 2020 Jun 22;2020.06.22.165225. 2,Adv Exp Med Biol. 2020;1231:45-51.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CXCL9 mAb against human CXCL9 (AA: 23-125) recombinant protein. (Expected MW is 37.7 kDa)
Western Blot
Figure 3:Western blot analysis using CXCL9 mAb against HEK293-6e (1) and human CXCL9 (AA: 23-125)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of HL-60 cells using CXCL9 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using CXCL9 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using CXCL9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.