Mouse Monoclonal Antibody to CXCL9
Item Information
Catalog #
Size
Price
Description
This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded is thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Aug 2020]
Product Overview
Entrez GenelD
4283
Aliases
CMK; MIG; Humig; SCYB9; crg-10
Clone#
7E4A10
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human CXCL9 (AA: 23-125) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Review Adv Exp Med Biol. 2020;1231:45-51. 2,bioRxiv. 2020 Jun 22;2020.06.22.165225.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f4306a8d25ac1fc967c9ffc854225a84a25b83853c58641d6701925ae9a5ff29.jpg?v=1666705746)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CXCL9 mAb against human CXCL9 (AA: 23-125) recombinant protein. (Expected MW is 37.7kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e99ca3c284d48ee2c96d2a9db2146db377d684656f118720bcac287feb4ef4c7.jpg?v=1666705746)
Figure 2:Western blot analysis using CXCL9 mAb against human CXCL9 (AA: 23-125) recombinant protein. (Expected MW is 37.7kDa)
Western Blot
![Figure 3:Western blot analysis using CXCL9 mAb against HEK293-6e (1) and CXCL9 (AA: 23-125)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/775e32cd29f7765ad3f91b90187a49114b9b646b94c014b755f727a1ad615960.jpg?v=1666705746)
Figure 3:Western blot analysis using CXCL9 mAb against HEK293-6e (1) and CXCL9 (AA: 23-125)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of K562 cells using CXCL9 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/2b56ab832890e106a1cb5d5d812d49bab10ef68bd94cbef1d4c75a02a6c76cf6.jpg?v=1666705746)
Figure 4:Flow cytometric analysis of K562 cells using CXCL9 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of THP-1 cells using CXCL9 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/2c79bb8fcb4bd2dcff298106d7f3a03e5ba44aec2547f9e08f8850f5673d0d4b.jpg?v=1666705746)
Figure 5:Flow cytometric analysis of THP-1 cells using CXCL9 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.