CTNNBL1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a component of the pre-mRNA-processing factor 19-cell division cycle 5-like (PRP19-CDC5L) protein complex, which activates pre-mRNA splicing and is an integral part of the spliceosome. The encoded protein is also a nuclear localization sequence binding protein, and binds to activation-induced deaminase and is important for antibody diversification. This gene may also be associated with the development of obesity. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Jul 2013]

Product Overview

Entrez GenelD

56259

Aliases

NAP; P14L; PP8304; C20orf33; dJ633O20.1

Clone#

3C7F9

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human CTNNBL1 (AA: 390-557) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/50 - 1/200

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Exp Cell Res. 2017 Aug 1;357(1):124-134.
2.Transl Psychiatry. 2014 Sep 30;4:e454.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CTNNBL1 mAb against human CTNNBL1 (AA: 390-557) recombinant protein. (Expected MW is 45.8 kDa)

Western Blot

Figure 3:Western blot analysis using CTNNBL1 mAb against HEK293 (1) and CTNNBL1 (AA: 390-557)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CTNNBL1 mouse mAb against Hela (1) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using CTNNBL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric