CTNNB1 Primary Antibody
Item Information
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Size
Price
Description
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
1499
Aliases
CTNNB; MRD19; armadillo
Clone#
7C5D2
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CTNNB1 (AA: 1-100) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Anticancer Res. 2016 Apr;36(4):1599-604.
2.Int J Clin Exp Pathol. 2015 Nov 1;8(11):14989-94.
2.Int J Clin Exp Pathol. 2015 Nov 1;8(11):14989-94.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CTNNB1 mAb against human CTNNB1 (AA: 1-100) recombinant protein. (Expected MW is 37.1 kDa)
Western Blot
Figure 3:Western blot analysis using CTNNB1 mAb against HEK293 (1) and CTNNB1 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CTNNB1 mouse mAb against A431 (1), U251 (2), and HEK293 (3) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of K562 cells using CTNNB1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CTNNB1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CTNNB1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.