cTnI Primary Antibody
Item Information
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Description
cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin), thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.
Product Overview
Entrez GenelD
7137
Aliases
CMH7; RCM1; cTnI; CMD2A; TNNC1
Clone#
3A10A12
Species Reactivity
Human
Immunogen
Purified recombinant fragment of cTnI expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Cummins B and Cummins P, J Mol Cell Cardiol, 1987, 19(10):999-1010.
2. Cummins B, Auckland ML, and Cummins P, Am Heart J, 1987, 113(6):1333-44.
3. Darnell J, Lodish H, and Baltimore D, Molecular Cell Biology, New York, NY: Scientific American Books, 1986, 827-8.
4. Larue C, Defacque-Lacquemant H, Calzolari C, et al, Mol Immunol, 1992, 29(2):271-8.
5. Adams JE III, Bodor GS, Davila-Roman VG, et al, Circulation, 1993, 88(1):101-6.
2. Cummins B, Auckland ML, and Cummins P, Am Heart J, 1987, 113(6):1333-44.
3. Darnell J, Lodish H, and Baltimore D, Molecular Cell Biology, New York, NY: Scientific American Books, 1986, 827-8.
4. Larue C, Defacque-Lacquemant H, Calzolari C, et al, Mol Immunol, 1992, 29(2):271-8.
5. Adams JE III, Bodor GS, Davila-Roman VG, et al, Circulation, 1993, 88(1):101-6.
Product Image
Western Blot
Figure 1: Western blot analysis using cTnI mouse mAb against truncated cTnI recombinant protein.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human normal cardiac muscle tissue, showing cytoplasmic localization using cTnI mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.