CSPG4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Product Overview
Entrez GenelD
1464
Aliases
NG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
Clone#
7G4E5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CSPG4 (AA: 2247-2308) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Res. 2011 Dec 15;71(24):7410-22.
2.Pigment Cell Melanoma Res. 2011 Dec;24(6):1148-57.
2.Pigment Cell Melanoma Res. 2011 Dec;24(6):1148-57.
Product Image
Western Blot
Figure 1: Western blot analysis using CSPG4 mAb against human CSPG4 recombinant protein. (Expected MW is 32.5 kDa)
Western Blot
Figure 2: Western blot analysis using CSPG4 mAb against HEK293 (1) and CSPG4 (AA: 2247-2308)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HEK293 cells using CSPG4 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.