CSPG4 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesNG2; MCSP; MCSPG; MSK16; HMW-MAA; MEL-CSPG
ProductOrderC
Clone#7G4E5
Entrez GenelD1464
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CSPG4 (AA: 2247-2308) expressed in E. Coli.
MW250.0kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
N
Catalog#: 30498
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Images
Western Blot
Figure 1: Western blot analysis using CSPG4 mAb against human CSPG4 recombinant protein. (Expected MW is 32.5 kDa)
Figure 1: Western blot analysis using CSPG4 mAb against human CSPG4 recombinant protein. (Expected MW is 32.5 kDa)
Western Blot
Figure 2: Western blot analysis using CSPG4 mAb against HEK293 (1) and CSPG4 (AA: 2247-2308)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using CSPG4 mAb against HEK293 (1) and CSPG4 (AA: 2247-2308)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HEK293 cells using CSPG4 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of HEK293 cells using CSPG4 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

References (references)
References (references)1.Cancer Res. 2011 Dec 15;71(24):7410-22.
2.Pigment Cell Melanoma Res. 2011 Dec;24(6):1148-57.