CRTC1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesMECT1; TORC1; WAMTP1; FLJ14027; KIAA0616; CRTC1
ProductOrderC
Clone#1B5
Entrez GenelD23373
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CRTC1 expressed in E. Coli.

MW67kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-353
Catalog#: 30075
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Images
Western Blot
Figure 1: Western blot analysis using CRTC1 mouse mAb against CRTC1(AA: 1-353)-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using CRTC1 mouse mAb against CRTC1(AA: 1-353)-hIgGFc transfected HEK293 cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of U251 (left) and NTERA2 (right) cells using CRTC1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Immunofluorescence analysis of U251 (left) and NTERA2 (right) cells using CRTC1 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of K562 cells using CRTC1 mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of K562 cells using CRTC1 mouse mAb (green) and negative control (purple).
Product Overview
Description

MECT1 (also known as MucoEpidermoid Carcinoma Translocated 1) functions as a transcriptional coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites. MECT1 does not appear to modulate CREB1 DNA-binding activity but enhances the interaction of CREB1 with TAF4/TAFII-130. MECT1 translocates with MAML2 (MasterMind-Like Protein 2) to yield a fusion oncogene: t(11;19) (q21;p13). This translocation occurs in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. The novel fusion product that results disrupts the Notch signaling pathway. The fusion protein consists of the N-terminus of MECT1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of MECT1 has been detected in a small number of mucoepidermoid carcinomas. Multiple isoforms have been reported for the MECT1 protein. Tissue specificity: Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.

References (references)
References (references)1. Genes Chromosomes Cancer. 2007 Jun;46(6):559-63.
2. Cancer Genet Cytogenet. 2008 Jan 15;180(2):135-9.
3. Biosci Rep. 2009 Apr;29(2):77-87.