CRKL Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Product Overview
Entrez GenelD
1399
Aliases
CRKL
Clone#
5E12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CRKL expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Mol Cell Biol. 2009 Jun;29(11):3076-87.
2. Cell. 2009 Jul 23;138(2):389-403.
2. Cell. 2009 Jul 23;138(2):389-403.
Product Image
Western Blot
Figure 1: Western blot analysis using CRKL mAb against human CRKL (AA: 100-303) recombinant protein. (Expected MW is 60 kDa)
Flow cytometric
Figure 2: Flow cytometric analysis of NIH/3T3 cells using CRKL mouse mAb (blue) and negative control (red).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.