CREB1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)

Product Overview

Entrez GenelD

1385

Aliases

CREB; MGC9284; CREB1

Clone#

5G3

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Purified recombinant fragment of human CREB1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Proc Natl Acad Sci U S A. 2008 Jul 22;105(29):10161-6.
2. FEBS Lett. 2008 Jun 11;582(13):1889-93.3
3. Am J Med Genet B Neuropsychiatr Genet. 2008 Jun 5;147B(4):500-4.

Product Image

Western Blot

Figure 1: Western blot analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), Hela (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (left) and submaxillary tumor tissues (right) using CREB1 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using CREB1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Elisa