CLL1 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signaling, glycoprotein turnover, and roles in inflammation and immune response. The protein encoded by this gene is a negative regulator of granulocyte and monocyte function. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. This gene is closely linked to other CTL/CTLD superfamily members in the natural killer gene complex region on chromosome 12p13.

Product Overview
Entrez GenelD
160364
Aliases
CLEC12A; MICL; CD371; CLL-1; DCAL-2
Clone#
4D5A8
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CLL1 (AA: extra 65-265) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Immunol. 2016 Oct 1;197(7):2715-25.
2.Eur J Immunol. 2006 Aug;36(8):2159-69.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CLL1 mAb against human CLL1 (AA: extra 65-265) recombinant protein. (Expected MW is 49.7 kDa)
Figure 2:Western blot analysis using CLL1 mAb against human CLL1 (AA: extra 65-265) recombinant protein. (Expected MW is 49.7 kDa)
Western Blot
Figure 3:Western blot analysis using CLL1 mAb against HEK293 (1) and CLL1 (AA: extra 65-265)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CLL1 mAb against HEK293 (1) and CLL1 (AA: extra 65-265)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using CLL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using CLL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of HL-60 cells using CLL1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HL-60 cells using CLL1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.