CLGN Primary Antibody
Item Information
Catalog #
Size
Price
Description
Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility.
Product Overview
Entrez GenelD
1047
Clone#
1C8B6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CLGN (AA: 249-405) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Arch Histol Cytol. 1999 Aug;62(3):283-93.
2. Dev Biol. 2001 Dec 1;240(1):254-61.
2. Dev Biol. 2001 Dec 1;240(1):254-61.
Product Image
Western Blot
Figure 1: Western blot analysis using CLGN mAb against human CLGN recombinant protein. (Expected MW is 43.5 kDa)
Western Blot
Figure 2: Western blot analysis using CLGN mAb against HEK293 (1) and CLGN (AA: 249-405)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using CLGN mouse mAb against LNCAP cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using CLGN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CLGN mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CLGN mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.