Mouse Monoclonal Antibody to CLEC5A

Item Information
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Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined. [provided by RefSeq, Jul 2008]

Product Overview
Entrez GenelD
23601
Aliases
MDL1; MDL-1; CLECSF5
Clone#
1B5G12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CLEC5A (AA: extra(28-188)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Biochem Biophys Res Commun. 2020 Apr 9;524(3):656-662. 2,Review Adv Exp Med Biol. 2020;1204:57-73.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CLEC5A mAb against human CLEC5A (AA: extra(28-188)) recombinant protein. (Expected MW is 40kDa)
Figure 2:Western blot analysis using CLEC5A mAb against human CLEC5A (AA: extra(28-188)) recombinant protein. (Expected MW is 40kDa)
Western Blot
Figure 3:Western blot analysis using CLEC5A mAb against HEK293-6e (1) and human CLEC5A (AA: extra(28-188))-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CLEC5A mAb against HEK293-6e (1) and human CLEC5A (AA: extra(28-188))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CLEC5A mouse mAb against THP-1 (1) cell lysate.
Figure 4:Western blot analysis using CLEC5A mouse mAb against THP-1 (1) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of U937 cells using CLEC5A mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of U937 cells using CLEC5A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.