Mouse Monoclonal Antibody to CLEC5A
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
23601
Aliases
MDL1; MDL-1; CLECSF5
Clone#
1B5G12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CLEC5A (AA: extra(28-188)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Biochem Biophys Res Commun. 2020 Apr 9;524(3):656-662. 2,Review Adv Exp Med Biol. 2020;1204:57-73.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7020d9f46d64a1b40f14d1f6014029a44156f530a74ed4ebe755d4d71a2f2d38.jpg?v=1666705773)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CLEC5A mAb against human CLEC5A (AA: extra(28-188)) recombinant protein. (Expected MW is 40kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a7dc528ce82a5116695153c75b3e7fb641b8227377479c0114f6278fee29efdd.jpg?v=1666705773)
Figure 2:Western blot analysis using CLEC5A mAb against human CLEC5A (AA: extra(28-188)) recombinant protein. (Expected MW is 40kDa)
Western Blot
![Figure 3:Western blot analysis using CLEC5A mAb against HEK293-6e (1) and human CLEC5A (AA: extra(28-188))-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d54878e884fde0f5b91a910ad5d5409ef6b4d997074c9f805248d65f1fbfa2de.jpg?v=1666705773)
Figure 3:Western blot analysis using CLEC5A mAb against HEK293-6e (1) and human CLEC5A (AA: extra(28-188))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using CLEC5A mouse mAb against THP-1 (1) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/06b9dd1055dfb9d90f4e8ec99c7e22ac255d4151c2be6c1fb084420f835cfc72.jpg?v=1666705773)
Figure 4:Western blot analysis using CLEC5A mouse mAb against THP-1 (1) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of U937 cells using CLEC5A mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/70048834cdafa6c3e8928eeb91054b99d01f5f3d35a3d3455e469dab510280a0.jpg?v=1666705773)
Figure 5:Flow cytometric analysis of U937 cells using CLEC5A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.